Relationship between dhea and dhea-s with anthropometric indices in women with different grades of obesity

Dehydroepiandrosterone [DHEA] and dehydroepiandrosterone sulfate [DHEA-S] are the most abundant steroids in human plasma. The aim of this study was to evaluate the relationship between DHEA and DHEA-S and anthropometric indices in women with different grades of obesity. This cross- sectional study investigated 170 women; 35 normal weight [BMI= 18.9-24.9], 33 overweight, [BMI = 25-29.9] as 36 women [BMI= 30-34.9] as obese grade I, 33 [BMI = 35-39.9] as obese grade II and 33 [BMI>40] as obese grade III. Body mass index was defined as weight in kilograms divided by the square of the height in meters. Serum levels of dehydroepiandrosterone, dehydroepiandrosterone sulfate and glucose were measured by commercially available enzyme immunoassay kits and the glucose oxidase method, respectively. There was a negative and significant correlation between DHEA and age in the normal [r=-0.457, P=0.006] overweight [r=-0.414, P=0.017] obese l [r=-0.402, P=0.015] obese ll [r=-0.391, P=0.024] and obese III [r=-0.354, P=0.043] groups, respectively. Also a negative and significant correlation was found between DHEA-S and age in overweight [r=-0.394, P=0.019], obese grade I [r=-0.455, P=0.005] and obese grade II [r=-0.390, P=0.023] groups respectively. We found a positive and significant correlation between DHEA and frame size in individuals of the Obese I, Obese II and Obese III groups, and also a positive and significant correlation between DHEA-S and frame sizes in individuals of these three groups. Results showed that serum levels of DHEA decrease with increasing grades of obesity, whereas serum levels of DHEA-S increase with increasing obesity

[Changes of serum leptin levels in healthy women with different grades of obesity and its correlation with hormonal and anthropometric factors]

Obesity is associated with a number of endocrine and metabolic abnormalities. Leptin is a peptide that is strongly correlated with adiposity and is a potential determinant of obesity and its complications. The aim of this study was to evaluate the relationship between serum leptin levels with anthropometric and hormonal factors in healthy women with different grades of obesity. This cross- sectional study enrolled 38 women with BMI ranging between 18.9-24.9, as the normal weight group, 35 women with BMI 24.9-29.9, as overweight, 37 women with 29.9-34.9, as obese grade I and 34 women with BMI 34.9-39.9, as obese grade II. Body Mass Index was defined as the weight in kilograms divided by the square of the height in meters. Serum levels of dehydroepiandrosterone sulfate, insulin, cortisol and Leptin were measured by commercially available enzyme immunoassay kits. There was a dramatic, continuous increase in serum leptin concentration when the degree of obesity was increased and concordance was seen with serum insulin concentrations. There was a direct and significant correlation between serum leptin concentration and BMI in obese subjects[r= 0.736, P< 0.001]. We found significant correlation between dehydroepiandrosterone sulfate concentrations and cortisol [r= 0.237, P< 0.05]. There was a significant negative correlation between leptin and insulin in grade 2 obese subjects[r= - 0.566, p< 0.05] and a significant positive correlation between cortisol and dehydroepiandrosterone sulfate in grade 2 obese subjects [r=0.610, P<0.001]. Serum leptin levels continuously rose with increasing degrees of obesity and serum leptin concentrations were strongly correlated with BMI. Concentrations of insulin and cortisol increase with increasing serum leptin levels

[Comparison of serum leptin levels of non-diabetic and diabetic obese subjects and its relationship to anthropometric indices]

Leptin, the product of the ob gene, plays a significant role in the pathogenesis of obesity and type 2 diabetes mellitus. The aim of this study was to compare serum leptin level in non-diabetic and type 2 diabetic obese subjects and assess its relationship to anthropometric indices. This cross-sectional study was performed on 35 obese subjects with type 2 diabetes and 35 non-obese, non-diabetics. Fasting lipid profiles were measured using enzymatic methods. The NycoCard HbA1c Kit was used to measure HbA1c. Serum leptin, insulin and glucose levels were measured by an enzyme immunoassay, using a commercially available kit and glucose oxidase methods respectively. The insulin resistance index was calculated by homeostasis model assessment [HOMA-IR]. The mean of insulin resistance index [HOMA-IR], HbA1c, diastolic blood pressure, triglyceride and fasting glucose in diabetics were significantly higher than in non-diabetic subjects [P < 0.05]. Serum leptin levels were significantly lower in diabetics than in non-diabetics [21.51 +/- 2.18 vs. 30.36 +/- 2.46] and were significantly higher in women than in men [31.85 +/- 17.96 vs.12.80 +/- 9.02] in the diabetic and [36.11 +/- 10.99 vs. 23.55 +/- 15.72] in non-diabetic groups. There was a direct and significant correlation between serum leptin levels with hip circumference [r= 0.450, p= 0.04] in diabetics and [r=0.590, p=0.000] in non-diabetics, and between leptin and BMI [r= 0.666, p= 0.000] in diabetic and [r= 0.490, p= 0.003] in non-diabetic groups. Since the mean serum leptin level is lower in obese diabetes, compared to non-diabetics, further studies are required to clarify the mechanisms of lower leptin levels in obese diabetic subjects